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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Pesca e Aquicultura. |
Data corrente: |
03/11/2021 |
Data da última atualização: |
04/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ADOLFI, M. C.; DU, K.; KNEITZ, S.; CABAU, C.; ZAHM, M.; KLOPP, C.; FERON, R.; PAIXÃO, R. V.; VARELA, E. S.; O'SULLIVAN, F. L. A.; OLIVEIRA, M. A. de; NÓBREGA, R. H.; LOPEZ-ROQUES, C.; IAMPIETRO, C.; LLUCH, J.; KLOAS, W.; WUERTZ, S.; SCHAEFER, F.; STÖCK, M.; GUIGUEN, Y.; SCHARTL, M. |
Afiliação: |
MATEUS C. ADOLFI, University of Wuerzburg; KANG DU, University of Wuerzburg; SUSANNE KNEITZ, University of Wuerzburg; CÉDRIC CABAU, Université de Toulouse; MARGOT ZAHM, Université de Toulouse; CHRISTOPHE KLOPP, INRA; ROMAIN FERON, INRAE; RÔMULO V. PAIXÃO, Bolsista CPAA; EDUARDO SOUSA VARELA, CNPASA; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA; MARCOS A. DE OLIVEIRA, UNESP; RAFAEL H. NÓBREGA, UNESP; CÉLINE LOPEZ-ROQUES, INRAE; CAROLE IAMPIETRO, INRAE; JÉRÔME LLUCH, INRAE; WERNER KLOAS, Institute of Freshwater Ecology and Inland Fisheries; SVEN WUERTZ, Institute of Freshwater Ecology and Inland Fisheries; FABIAN SCHAEFER, Institute of Freshwater Ecology and Inland Fisheries; MATTHIAS STÖCK, Institute of Freshwater Ecology and Inland Fisheries; YANN GUIGUEN, INRAE; MANFRED SCHARTL, Comprehensive Cancer Center Mainfranken. |
Título: |
A duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas). |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Scientific Reports, v. 11, art. 21544, 2021. |
ISSN: |
2045-2322 |
DOI: |
https://doi.org/10.1038/s41598-021-01066-z |
Idioma: |
Inglês |
Conteúdo: |
Arapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF B signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes. |
Thesagro: |
Cromossoma; Peixe; Pirarucu. |
Thesaurus Nal: |
Arapaima gigas; Chromosomes; Cytogenetics; Fish; Sex chromosomes; Sex determination. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227368/1/Arapaima-sexmaster-gene-2021.pdf
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Marc: |
LEADER 02743naa a2200493 a 4500 001 2135766 005 2021-11-04 008 2021 bl uuuu u00u1 u #d 022 $a2045-2322 024 7 $ahttps://doi.org/10.1038/s41598-021-01066-z$2DOI 100 1 $aADOLFI, M. C. 245 $aA duplicated copy of id2b is an unusual sex-determining candidate gene on the Y chromosome of arapaima (Arapaima gigas).$h[electronic resource] 260 $c2021 520 $aArapaima gigas is one of the largest freshwater fish species of high ecological and economic importance. Overfishing and habitat destruction are severe threats to the remaining wild populations. By incorporating a chromosomal Hi-C contact map, we improved the arapaima genome assembly to chromosome-level, revealing an unexpected high degree of chromosome rearrangements during evolution of the bonytongues (Osteoglossiformes). Combining this new assembly with pool-sequencing of male and female genomes, we identified id2bbY, a duplicated copy of the inhibitor of DNA binding 2b (id2b) gene on the Y chromosome as candidate male sex-determining gene. A PCR-test for id2bbY was developed, demonstrating that this gene is a reliable male-specific marker for genotyping. Expression analyses showed that this gene is expressed in juvenile male gonads. Its paralog, id2ba, exhibits a male-biased expression in immature gonads. Transcriptome analyses and protein structure predictions confirm id2bbY as a prime candidate for the master sex-determiner. Acting through the TGF B signaling pathway, id2bbY from arapaima would provide the first evidence for a link of this family of transcriptional regulators to sex determination. Our study broadens our current understanding about the evolution of sex determination genetic networks and provide a tool for improving arapaima aquaculture for commercial and conservation purposes. 650 $aArapaima gigas 650 $aChromosomes 650 $aCytogenetics 650 $aFish 650 $aSex chromosomes 650 $aSex determination 650 $aCromossoma 650 $aPeixe 650 $aPirarucu 700 1 $aDU, K. 700 1 $aKNEITZ, S. 700 1 $aCABAU, C. 700 1 $aZAHM, M. 700 1 $aKLOPP, C. 700 1 $aFERON, R. 700 1 $aPAIXÃO, R. V. 700 1 $aVARELA, E. S. 700 1 $aO'SULLIVAN, F. L. A. 700 1 $aOLIVEIRA, M. A. de 700 1 $aNÓBREGA, R. H. 700 1 $aLOPEZ-ROQUES, C. 700 1 $aIAMPIETRO, C. 700 1 $aLLUCH, J. 700 1 $aKLOAS, W. 700 1 $aWUERTZ, S. 700 1 $aSCHAEFER, F. 700 1 $aSTÖCK, M. 700 1 $aGUIGUEN, Y. 700 1 $aSCHARTL, M. 773 $tScientific Reports$gv. 11, art. 21544, 2021.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
15/06/2020 |
Data da última atualização: |
01/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
FONTENELE, R. S.; SALYWON, A. M.; MAJURE, L. C.; COBB, I. N.; BHASKARA, A.; AVALOS-CALLEROS, J. A.; ARGÜLLO-ASTORGA, G. R.; SCHMIDLIN, K.; KHALIFEH, A.; SMITH, K.; SCHRECK, J.; LUND, M. C.; KÖHLER, M.; WOJCIECHOWSKI, M. F.; HODGSON, W. C.; PUENT-MARTINEZ, R.; VAN DOORSLAER, K.; KUMARI, S.; VERNIÈRE, C.; FILLOUX, D.; ROUMAGNAC, P.; LEFEUVRE, P.; RIBEIRO, S. G.; KRABERGER, S.; MARTIN, D. P.; VARSANI, A. |
Afiliação: |
RAFAELA S. FONTENELE, ARIZONA STATE UNIVERSITY, USA; ANDREW M. SALYWON, DESERT BOTANICAL GARDEN, USA; LUCAS C. MAJURE, DESERT BOTANICAL GARDEN, USA; ILARIA N. COBB, ARIZONA STATE UNIVERSITY, USA; AMULYA BHASKARA, ARIZONA STATE UNIVERSITY, USA; JESÚS A. AVALOS-CALLEROS, INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA, MEXICO; GERARDO R. ARGÜLLO-ASTORGA, INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA, MEXICO; KARA SCHMIDLIN, ARIZONA STATE UNIVERSITY, USA; ANTHONY KHALIFEH, ARIZONA STATE UNIVERSITY, USA; KENDAL SMITH, ARIZONA STATE UNIVERSITY, USA; JOSHUA SCHRECK, ARIZONA STATE UNIVERSITY, USA; MICHAEL C. LUND, ARIZONA STATE UNIVERSITY, USA; MATIAS KÖHLER, UFRGS; MARTIN F. WOJCIECHOWSKI, ARIZONA STATE UNIVERSITY, USA; WENDY C. HODGSON, DESERT BOTANICAL GARDEN, USA; RAUL PUENT-MARTINEZ, DESERT BOTANICAL GARDEN, USA; KOENRAAD VAN DOORSLAER, UNIVERSITY OF ARIZONA, USA; SAFAA KUMARI, ICARDA; CHRISTIAN VERNIÈRE, CIRAD, FRANCE; DENIS FILLOUX, CIRAD, FRANCE; PHILIPPE ROUMAGNAC, CIRAD, FRANCE; PIERRE LEFEUVRE, CIRAD, FRANCE; SIMONE DA GRACA RIBEIRO, Cenargen; SIMONA KRABERGER, ARIZONA STATE UNIVERSITY, USA; DARREN P. MARTIN, UNIVERSITY OF CAPE TOWNM, SOUTH AFRICA; ARVIND VARSANI, ARIZONA STATE UNIVERSITY, USA. |
Título: |
A novel divergent geminivirus identified in Asymptomatic new world Cactaceae plants. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Viruses, v. 12, n. 4, article 398, 2020. |
DOI: |
10.3390/v12040398 |
Idioma: |
Inglês |
Palavras-Chave: |
Cactoideae; Cochineal insects; Geminivirus; Opuntioideae; SsDNA virus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213975/1/viruses-12-00398.pdf
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Marc: |
LEADER 01308naa a2200481 a 4500 001 2123276 005 2020-12-01 008 2020 bl uuuu u00u1 u #d 024 7 $a10.3390/v12040398$2DOI 100 1 $aFONTENELE, R. S. 245 $aA novel divergent geminivirus identified in Asymptomatic new world Cactaceae plants.$h[electronic resource] 260 $c2020 653 $aCactoideae 653 $aCochineal insects 653 $aGeminivirus 653 $aOpuntioideae 653 $aSsDNA virus 700 1 $aSALYWON, A. M. 700 1 $aMAJURE, L. C. 700 1 $aCOBB, I. N. 700 1 $aBHASKARA, A. 700 1 $aAVALOS-CALLEROS, J. A. 700 1 $aARGÜLLO-ASTORGA, G. R. 700 1 $aSCHMIDLIN, K. 700 1 $aKHALIFEH, A. 700 1 $aSMITH, K. 700 1 $aSCHRECK, J. 700 1 $aLUND, M. C. 700 1 $aKÖHLER, M. 700 1 $aWOJCIECHOWSKI, M. F. 700 1 $aHODGSON, W. C. 700 1 $aPUENT-MARTINEZ, R. 700 1 $aVAN DOORSLAER, K. 700 1 $aKUMARI, S. 700 1 $aVERNIÈRE, C. 700 1 $aFILLOUX, D. 700 1 $aROUMAGNAC, P. 700 1 $aLEFEUVRE, P. 700 1 $aRIBEIRO, S. G. 700 1 $aKRABERGER, S. 700 1 $aMARTIN, D. P. 700 1 $aVARSANI, A. 773 $tViruses$gv. 12, n. 4, article 398, 2020.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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